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Promega checkmate mammalian two-hybrid system
Checkmate Mammalian Two Hybrid System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/checkmate+mammalian+two-hybrid+system/checkmate+mammalian+two+hybrid+system/bio_rxiv__2025__02__03__636272-70-4-1
Average 90 stars, based on 1 article reviews
checkmate mammalian two-hybrid system - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

In Vitro:

Article Title: Mutagenesis and functional analysis of the varicella-zoster virus portal protein.
Article Snippet: Herpesviruses replicate by cleaving concatemeric dsDNA into single genomic units that are packaged through an oligomeric portal present in preformed procapsids.. In contrast to what is known about phage portal proteins, details concern ing herpesvirus portal structure and function are not as well understood.. A panel of 65 Varicella-Zoster virus (VZV) recombinant portal proteins with five amino acid in-frame insertions were generated by random transposon mutagenesis of the VZV portal gene, ORF54.

Mutagenesis:

Article Title: Mutagenesis and functional analysis of the varicella-zoster virus portal protein.
Article Snippet: Herpesviruses replicate by cleaving concatemeric dsDNA into single genomic units that are packaged through an oligomeric portal present in preformed procapsids.. In contrast to what is known about phage portal proteins, details concern ing herpesvirus portal structure and function are not as well understood.. A panel of 65 Varicella-Zoster virus (VZV) recombinant portal proteins with five amino acid in-frame insertions were generated by random transposon mutagenesis of the VZV portal gene, ORF54.

Clone Assay:

Article Title: Mutagenesis and functional analysis of the varicella-zoster virus portal protein
Article Snippet: OPTITN83-Bind , Codon-optimized TN83 cloned into pBIND DNA binding domain vector , This study; Checkmate mammalian Two-Hybrid System (Promega). .. OPTITN83-Act , Codon-optimized TN83 cloned into pACT activation domain vector , This study; Checkmate mammalian Two-Hybrid System (Promega). .. pFastBac/CT , Used to clone ORF54 genes into baculovirus expression system , Invitrogen Life Sciences.

Activation Assay:

Article Title: Mutagenesis and functional analysis of the varicella-zoster virus portal protein
Article Snippet: OPTITN83-Bind , Codon-optimized TN83 cloned into pBIND DNA binding domain vector , This study; Checkmate mammalian Two-Hybrid System (Promega). .. OPTITN83-Act , Codon-optimized TN83 cloned into pACT activation domain vector , This study; Checkmate mammalian Two-Hybrid System (Promega). .. pFastBac/CT , Used to clone ORF54 genes into baculovirus expression system , Invitrogen Life Sciences.

Plasmid Preparation:


other:

Article Title: ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca.
Article Snippet: NLR inflammasomes recognize pathogen-associated molecular patterns (PAMPs), triggering Caspase-1 activation and leading to gasdermin D (GSDMD)-mediated pyroptosis, a crucial immune response in mammals.. The functional GSDME-mediated pyroptosis has been reported in invertebrates, yet the existence of an NLR-CaspaseGSDME axis mediating pyroptosis signaling cascades remains unclear.. In this study, we reported an NLRC4 homolog named ChNLRC4, a pattern recognition receptor from the oyster Crassostrea hongkongensis that is able to bind to LPS and Lys-type PGN through its LRR domain.



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